ROLE OF TAMM-HORSFALL PROTEIN (THP) IN LITHOGENIC PROCESS : ANIMAL STUDIES

Varsha Choudhary, Jyoti Bhardwaj, P. P. Singh

Abstract


One of the defenses against nephrolithiasis is provided by macromolecules that modulate the nucleation, growth,
aggregation and retention of crystals in the kidneys. According to its well-known physico-chemical properties. THP has a
dual role in modifying crystal aggregation: at high pH and low ionic strength (IS), THP is a powerful crystal aggregation
inhibitor. Upon lowering pH and rasing ionic strength THP viscosity increases, leading to reduced crystal aggregation
inhibition.For this purpose eight guinea pigs were made hyperoxaluric.The treatment was given for fifteen days;then
urine samples were collected before treatment ; then on 5,10,15and 25 day( after treatment) and in vitro addition of THP
on 30th day which was isolated from hyperoxaluric and normal animals.The effect of EG+GM on urinary oxalate,THP and
TBAR levels increases but after treatment the urine chemistry revert to normal profile,though plasma TBAR levels were
appreciably high.The crystallization of calcium was almost double when THP was isolated from hyperoxaluric animals
rather than normals.Our study suggested that THP act as a promoter.


Keywords


Nephrolithiasis;calcium oxalate inhibitors and promoters;Tamm- Horsfall Protein.Ethylene glycol;gentamicin

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References


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