STUDY OF UROPATHOGENS AND ANTIBIOTIC SUSCEPTIBILITY IN CASES OF URINARY TRACT INFECTIONS (UTI) IN RAMA MEDICAL COLLEGE AND HOSPITAL, HAPUR.

Dr. Muzaffari Yasmeen, Dr. Md Rashid Taj

Abstract


Background: One of the most common infectious diseases diagnosed in developing countries is urinary tract infection (UTI). extensive exercise of antibiotics in opposition to uropathogens has led to the appearance of antibiotic resistant. A laboratory based cross-sectional survey was conducted in Rama Medical College and Hospital to determine the prevalence and antibiotic susceptibility of uropathogens in hapur district .

Methods: We have collected 101 clean catch mid-stream urine samples from all suspected UTI  using sterile screw capped container. The urine samples   were cultured and processed for subsequent uropathogens isolation. The identified species were then exposed to selected antibiotics to test for their susceptibility.

Results: The overall prevalence of urinary tract infection in the area was 60.39%. Most frequently isolated uropathogen in our study was Escherichia coli was the most prevalent bacterial uropathogen with 21/61 (34.5%) followed by Staphylococcus aureus 16/61 (26.2%), Klebsiella pneumoniae 12/61 (19.6%), Proteus mirabilis 1/61 (1.2%), Enterococcus faecalis 6/61(9.8%), and Proteus aeruginosa 2/61 (3.2%). Escherichia coli was mostly sensitive to Meropenam and Cefuroxime 17/21 (80.96%), Staphylococcus aureus highest sensitivity to piperacillin/tazobactam 14/16 (87.5%) . Sensitivity of Klebsiella pneumonia was good with most of the anti biotics  with best result of gatifloxacin 11/12 (91.67%). Enterococcus faecalis was sensitive to almost all the antibiotics having 100% sensitivity to antibiotics like vancomycin, meropenam and  piperacillin/tazobactam.

Conclusion: The option of empirical antimicrobial treatment is notably affected by uropathogen prevalences according to age and gender . judicious use of antibiotic is important

Keywords


Antibiotic sensitivity test, uropathogen, Urinary tract infection

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References


A. R. Ronald, L. E. Nicolle, E. Stamm et al., “Urinary tract infection in adults:

research priorities and strategies,” International Journal of Antimicrobial

Agents, vol. 17, no. 4, pp. 343–348, 2001.

Royal College of General Practitioners, Office of Population Censuses and

Surveys, Department of Health, Morbidity Statistics from General Practice:

Fourth National Study 1991- 1992, Series MB5, no. 3, HMSO, London, UK, 1995.

M. S. Litwin, C. S. Saigal, E. M. Yano et al., “Urologic diseases in America

project: analytical methods and principal findings,” Journal ofUrology, vol.

, no. 3, pp. 933–937, 2005.

M. Grabe, M. C. Bishop, and T. E. Bjerklund-Johansen, Guidelines on

Urological Infections, European Association of Urology, 2009.

Pires MCS, Frota KS, Martins Junior PO, Correia AF, Cortez-Escalante JJ,

Silveira CA. Prevalência e suscetibilida¬des bacterianas das infecções

comunitárias do trato urinário, em Hospital Universitário de Brasília, no

período de 2001 a 2005. Rev Soc Bras Med Trop 2007;40:643-7.

J. Sabrina, “Antimicrobial resistance among producers and non-producers of

extended spectrum beta-lactamases in urinary isolates at a tertiary Hospital

in Tanzania,” BMC Research Notes, vol. 3, p. 348, 2010.

Drekonja DM, Johnson JR. Ur inar y tract inf ections. Pr im Care.

;35:345–367.

Taneja N, Chatterjee SS, Singh M, Singh S, Sharma M. Pediatric urinary tract

infections in a tertiary care center from Northern India. Indian J Med Res.

;131:101–105.

Jeyaseelan EC, Jashothan PT. In vitro control of Staphylococcus aureus (NCTC

and Escherichia coli (ATCC 25922) by Ricinus communis L. Asian Pac J

Trop Biomed. 2012;2(9):717–721.

Haque R, Akter ML, Salam MA. Prevalence and susceptibility of

uropathogens: a recent report from a teaching hospital in Bangladesh. BMC

Research Notes. 2015;8(1):416.

Ayoade F, Moro DD, Ebene OL. Prevalence and antimicrobial susceptibility

pattern of asymptomatic urinary tract infections of bacterial and parasitic

origins among university students in redemption camp, Ogun state, Nigeria.

Open Journal of Medical Microbiology. 2013;03(04):8.

Flores-Mireles AL, et al. Urinary tract infections: epidemiology, mechanisms

of infection and treatment options. Nat Rev Micro. 2015;13(5):269–84.

Scholes D, et al. Risk factors for recurrent urinary tract infection in young

women. J Infect Dis. 2000;182(4):1177–82.

Nicolle LE. Urinary tract infections in the elderly. Clin Geriatr Med.

(3):423–36.

Shortliffe LM, McCue JD. Urinary tract infection at the age extremes:

pediatrics and geriatrics. Am J Med. 2002;113(Suppl 1A):55s–66s.

Ehinmidu JO. Antibiotics susceptibility patterns of urine bacterial isolates in

Zaria, Nigeria. Trop J Pharm Res. 2003;2(2):223–28.

Monali Priyadarsini Mishra et al./Asian Pac J Trop Biomed 2013; 3(4): 315-324

Ullah F, Malik S, Ahmed J. Antibiotic susceptibility pattern and ESBL

prevalence in nosocomial Escherichia Coli from urinary tract infections in

Pakistan. Afr J Biotechnol. 2009;8(16):3921–26.

Tesfahunegn Z, et al. Bacteriology of surgical site and catheter related urinary

tract infections among patients admitted in Mekelle hospital, Mekelle, Tigray,

Ethiopia. Ethiop Med J. 2009;47(2):117–27.

M. Odoki, J. Bazira, M. L. Moazam, and E. Agwu, “Healthpoint survey of

bacteria urinary tract infections among suspected diabetic patients

attending clinics in Bushenyi district of Uganda,” Special Bacterial Pathogens

Journal (SBPJ), vol. 1, no. 1, pp. 0005–0009, 2015.

P. A. Ekwealor, M. C. Ugwu, I. Ezeobi et al., “Antimicrobial evaluation of

bacterial isolates from urine specimen of patients with complaints of urinary

tract infections in Awka, Nigeria,” International Journal of Microbiology, vol.

, Article ID 9740273, 6 pages, 2016.

A. Baguma, K. Atek, and J. Bazira, “Prevalence of extendedspectrum betalactamases-

producing microorganisms in patients admitted at KRRH,

southwestern Uganda,” International Journal of Microbiology, vol. 2017,

Article ID 3183076, 5 pages, 2017.

L. K. Khanal, R. Shrestha, A. Barakoti, S. Timilsina, and R. Amatya, “Urinary tract

infection among males and females- a comparative study,” Nepal Medical

College Journal, vol. 18, no. 3-4, pp. 97–99, 2016.

D. S. Lo, H. H. Shieh, S. L. B. Ragazzi, V. H. K. Koch, M. B. Martinez, and A. E. Gilio,

“Community-acquired urinary tract infection: age and gender-dependent

etiology,” Jornal Brasileiro de Nefrologia, vol. 35, no. 2, pp. 93–95, 2013.

Peixoto De Miranda, E.J.; Salvador De Oliveira, G.S.; Roque, F.L.; Dos Santos,

S.R.; Olmos, R.D. & Lotufo, P.A. - Susceptibility to antibiotics in urinary tract

infections in a secondary care setting from 2005-2006 and 2010-2011, in São

Paulo, Brazil: data from 11,943 urine cultures. Rev. Inst. Med. Trop. Sao Paulo,

(4): 313-24, 2014

Nomura S, Hanaki H, Nagayama A Tazobactam-piperacillin compared with

sulbactam-ampicillin, clavulanic acid-ticarcillin, sulbactam-cefoperazone,

and piperacillin for activity against beta-lactamase-producing bacteria

isolated from patients with complicated urinary tract infections. J. Chemother

Apr;9(2):89-94


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