Aravindhan. G, Dr. S. S. M. Umamageswari, M. Mohana Priya


Urinary tract  infections (UTI) is a serious health problem with respect to antibiotic resistance and biofilm formation being the prime cause for antibiotic resistance. The biofilms produced by E.coli may be difficult to treat as they exhibit multidrug resistance. The present study is aimed to perform invitro detection of biofilm formation among E.coli strains from urine cultures. A total number of 180 E.coli strains were isolated from urine samples suffering from UTI. The samples were processed using standard phenotypic identification  techniques and tested for biofilm production by three methods congo red agar(CRA), tube method(TM) and tissue culture plate method(TCP). Among 180 E.coli isolates, 80 and 100 strains were collected from catheterized and non-catheterized patients respectively. In biofilm production 96, 97 and 119 were positive for biofilm productions by Congo red agar (CRA), Tube method(TM) and Tissue culture plate (TCP) method respectively. Among the biofilm producers maximum resistance was seen to cephalexin 168(93%), ampicillin 158(87%), norfloxacin 154(85%), ciprofloxacin154(85%),ceftazidime with clavulinic acid 146 (81%),gentamicin125(69%), piperacillin tazobactam 81(45%), cefoperazone  with sulbactam 79(43%). The antibiotic resistance among biofilm producing E.coli was found to be higher than that of non-biofilm with a p=0.01(<0.05)which is statistically significant. A greater understanding of the nature of biofilm  producing  E.coli in UTIs will help in the development of new and more effective treatment.   


Escherichia coli, antibiotic resistance, biofilm.

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