Nidhi Jain, Akanksha Mathur, Hemlata Verma


Objective: To study the bacteriological profile and the pattern of antibiotic sensitivity and resistance in pus culture isolates in a tertiary care
teaching hospital in Bhopal.
Materials and Methods: Pus specimens submitted to the microbiology laboratory for routine cultures and sensitivity were analyzed prospectively
for the duration of 2 months. Antimicrobial susceptibility testing was performed by Kirby-Bauer Disk Diffusion method.
Results: Among the total 440 samples analyzed, 319 samples (72.5%) were found to be positive for growth. Out of 319 growths, 286 shows single
isolate and 33 shows mixed (double) isolates. Out of these positive samples, 269 samples (84.33%) were positive for Gram negative bacteria while
44 samples (13.8%) were positive for Gram positive bacteria, 4 (1.25%)were positive for both and 2 samples (0.63%) were positive for growth
other than bacteria i.e. fungal growth. Klebsiella was the most common Gram–negative bacteria isolated & other common Gram-negative bacteria
isolated were Pseudomonas and E.coli species while Staphylococcus aureus & CONS were the commonest Gram–positive bacteria. Gram
negative bacteria shows >35% sensitivity to Meropenem, Imipenem-cilastatin, Cefoperazone and >20% sensitivity to Piperacillin-Tazobactam
and Gatifloxacin. They were most resistant to Cefpodoxime, Cefprozil. Gram positive bacteria were >70% sensitive to Lincomycin and
Ampicillin-Sulbactam while most resistant to Clarithromycin, Sparfloxacin, Gatifloxacin.
Conclusion: We must use antibiotics rationally and judiciously as these are precious and limited resources. So in order to combat the menace of
resistant microorganisms, we should join hands to formulate safe and effective antibiotic policies at loco-regional as well as national level.
Because, if such type of indiscriminate, irrational and widespread use of antibiotics is allowed to continue, the day is not far when the resistance
rates among the micro-organisms will become so high that the most innocuous looking infections may prove fatal for life.


Bacteriological profile, antibiotic resistance, sensitivity, pus.

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