BIODEGRADATION AND BIOREMEDIATION OF GLYPHOSATE HERBICIDE BY NOCARDIA MEDITERRANIE THS 1

Ajish. A, T. H Sukirtha

Abstract


Glyphosate herbicide is one of the main compounds used as a control /killing agent of a wide variety of wild grasses and other herbaceous plants. The bioremediation of these glyphosate affected soil depends upon the capability to isolate bacteria which has the capability to degrade these xenobiotic compounds. The capability of Nocardia mediterranie THS 1 strain to produce biosurfactants for degrading the herbicide glyphosate was studied by growing the organism in MS medium with glyphosate, the organisms capability to utilize the glyphosate as further proved by growing these organisms in MS medium incorporated with 3 different concentrations of glyphosate which is normally applied to the field . The study also proved that Nocardia mediterranie THS 1 had a high capability to degrade glyphosate at a higher rate as the OD values had a significant increase as the concentration increased from 10ppm to 30ppm. The GC-MS result also showed the degraded product of glyphosate that is AMPA in various fragmented peaks thus concluding that this strain could be used as a great bio controlling agents in various contaminated agricultural fields.


Keywords


Glyphosate, xenobiotics, Actinomycetes sp, Nocardia mediterranie THS 1, AMPA.

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References


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