Adhavan. I, Dr. P. A. T. Jagadeeshwari



      The Gram stain is a bacteriological laboratory technique used to differentiate between gram positive and gram negative bacteria. The most important primary test performed directly on clinical specimen is gram staining.

Aims and Objective:-

            To correlate the gram stain findings with the bacteria isolated from the sputum samples obtained from patients

Materials required:-

This is a retrospective study conducted for a period of 6 months (June 2018 and December 2018) at Department of Microbiology in Saveetha Medical College and Hospital, Chennai.


The sputum collected from 109 patients from different departments was sent to the microbiology laboratory and following data has been arrived.


1. Most common bacterial isolate from sputum was Pseudomonas aeruginosa.

2. The presence of pus cells between 10 to 25 had a better correlation than that of pus cells less than 10.

3. The epithelial cells had an inverse correlation with isolation of pathogen.


Gram stain, Klebsiella pneumonia, sputum, epithelial cells, pus cells.

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