Bhawani Shankar Verma, Dr Rachna, Mohammad Suhaib, Prasanna Gupta


Background: Bloodstream Infection (BSI) is a significant and important caused of morbidity and mortality worldwide. Illness associated with the BSIs range from self limiting infection to life threatening sepsis that required rapid intervention and the choice of antimicrobial therapy for BSI is often empirical and based on local antimicrobial therapy of the most common aetiologies.

Objective: Epidemiological profile of various bacterial isolates from blood sample and their antibiotic susceptibility pattern at a tertiary care hospital.

Methodology: A total 680 samples were collected from suspected patient with bloodstream infection from various IPD and OPD wards of NIMS. Blood sample with positive growth by BACTEC automated blood culture system and processed to microbiology standard laboratory method and then antibiotic sensitivity test was performed by using CLSI guidelines.

Result:  Out of 680 totals blood sample, 155(22.79%) were blood culture positive. Out of 155 positive sample 97(62.58%) were male while 58(37.42%) were female. Gram negative bacilli were 82(52.90%) while Gram positive cocci were 73(47.09%). Total isolates, the most predominant Gram negative organism was Klebsiella species 30(19.35%), while Gram positive cocci was MSSA (Staphylococcus aureus).  Most of the Gram negative cocci were susceptible to Meropenem and Gram positive cocci susceptible to Vancomycin and Linezolid.

Conclusion: Gram negative organism are most predominate in bloodstream infection. Increasing in antibiotic resistance for BSIs causing of all pathogens has been necessitated continuous monitoring of susceptibility pattern of organism to prevent and spread of drug resistance.


MSSA- Methicillin Sensitive Staphylococcus Aureus, BSIs- Bloodstream infection, Indoor Patient department, Outdoor patient department.

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