ROLE OF Ki67 IN ORAL PREMALIGNANT AND MALIGNANT SQUAMOUS CELL LESION

Dr. Mahendra Singh, Dr. Monika Verma

Abstract


Aims-To study Ki67 expression in oral premalignant lesion & its comparison with malignant lesion.

Material and methods- The standard immunohistochemical method along with MIB-1 DO-7;DAKO antibodies was use to study the expression of Ki67 in paraffin embedded tissue specimens.

Results- all sample study showed positive staining for ki67, only 9% case from oral squamous cell carcinoma(OSCC) group showed negative staining for ki67. The staining was confined to basal layer in most of the cases except OSCC in which it was seen in all layers. The intensity of staining was moderate to intense. The percentage of ki67 positive cells in malignant mucosa was 91%. The statistical analysis revealed that the expression of ki67 increases from dysplasia to malignancy.

Conclusion- these results emphasize the potential use of ki67 antigen as marker of malignant transformation and carcinogenesis in oral premalignant lesions, conditions and OSCC, respectively; and in future they may serve as prognostic tools in the early detection of malignant transformation in oral premalignant lesions and conditions.


Keywords


Ki67, oral squamous cell carcinoma(OSCC), premalignant lesion

Full Text:

PDF

References


Silverman S Jr, Gorsky M. Epidemiologic and demographic update in oral cancer: California and national data-1973to 1985. J Am Dent Assoc1990;120:523-4.

Tanaka T, Ishigamori R. Understanding Carcinogenesis for Fighting Oral Cancer. Journal of Oncology2011;603740:10.

Shafer WG, Hine MK, Levy BM. Benign and malignant tumors of oral cavity. In Rajendran R, Sivapathasundharan B (eds) A textbook of oral pathology5thed, Elseiver, New Dehli 2005;121-36.

Scholzen T, Gerdes J (March 2000). "The Ki-67 protein: from the known and the unknown". Journal of Cellular Physiology. 182 (3): 311–22.

Bruno S, Darzynkiewicz Z (January 1992). "Cell cycle dependent expression and stability of the nuclear protein detected by Ki-67 antibody in HL-60 cells". Cell Proliferation. 25 (1): 31–40. doi:10.1111/j.1365-2184.1992.tb01435.x. PMID 1540682.

Schonk DM, Kuijpers HJ, van Drunen E, van Dalen CH, Geurts van Kessel AH, Verheijen R, Ramaekers FC (October 1989). "Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10". Hum. Genet. 83 (3): 297–9. doi:10.1007/BF00285178. PMID 2571566.

Bullwinkel J, Baron-Lühr B, Lüdemann A, Wohlenberg C, Gerdes J, Scholzen T (March 2006). "Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells". J. Cell. Physiol. 206 (3): 624–35. doi:10.1002/jcp.20494. PMID 16206250.

R. Rahmanzadeh G. Hüttmann J. Gerdes T. Scholzen. Chromophore‐assisted light inactivation of pKi‐67 leads to inhibition of ribosomal RNA synthesis. Journal compilation © 2007 Blackwell Publishing Ltd, Cell Proliferation, 40, 422–430.

Mehrotra R, Singh M,Kumar D, Pandey AN, Gupta RK, Sinha US: Age specific incidence rate and pathological spectrum of oral cancer in Allahabad, Ind J Med Sci 2003, 57:400-404, 2003.

Reddy V., Saxena MS. Assessment of Ki67 in healthy, premalignant and malignant lesions of the oral mucosa and its predictive role in disease progression. Int J Oral-Med Sci 2010;9 (2):67-74.

Francesca A, Berenzi A, Benetti A, Rossi E, Sordo R D et al. Expression of P16, P53 and Ki-67 Proteins in the Progression of Epithelial Dysplasia of the Oral Cavity. ANTICANCER RESEARCH 2008;28: 2535-2540.

Miguel A. Gonzalez-Moles, Ruiz-Avila I, Rodriguez-Archilla A, Martinez-Lara I. Suprabasal expression of Ki-67 antigen as a marker for the presence and severity of oral epithelial dysplasia. Head and Neck. 2000;22:658-61.

Dwivedi N., Chandra S., KashyapB., Raj V., Agarwal A. Suprabasal expression of Ki-67 as a marker for the severity of oral epithelial dysplasia and oral squamous cell carcinoma.Contemp Clin Dent 2013;4:7-12

Birajdar SS,Radhika M B,Paremala K,Sudhakara M,Soumya M, Gadivan M. Expression of Ki67 in normal oral epithelium,leukoplakia oral epithelium and oral squamous cell carcinoma.J Oral Maxillofac Pathol 2014;18:169-76


Refbacks

  • There are currently no refbacks.