IN VITRO STUDY OF ANTIMICROBIAL ACTIVITY OF CALCIUM HYDROXIDE MIXED WITH DIFFERENT VEHICLES AGAINST E.FAECALIS AND CANDIDA ALBICANS

Dr. Noushad M. C,, Dr. Rakhi R, Dr. Shaheen Abootty, Dr. Kavya Maheesan, Dr. Suneetha M. P, Dr Ashraf. K

Abstract


Objective.  : The purpose of this in vitro study was to investigate antimicrobial activity of calcium hydroxide (CH)  in  combination  with  chlorhexidine gluconate (CHX),glycerine,  chitosan  and  saline  against Enterococcus faecalis and Candida albicans.

Study design: Antimicrobial activity was determined using agar diffusion test. Standard well  in the cultivated agar plates were filled with one of the calcium hydroxide preparations and control agents. The zones of microbial inhibition were measured after incubation period.

 Results. : The  combination  of  Calcium  hydroxide  with chlorhexidine  demonstrated  most  antibacterial  activity  of  all  other  preparations. All  the  tested  groups  had  significant  antimicrobial  action  against E faecalis  and  C albicans.

Conclusion : Antimicrobial  activity  of  Calcium  hydroxide  may change with  the  type  of  the  vehicle.


Keywords


Calcium hydroxide, chitosan, chlorhexidine, glycerine, antimicrobial activity, vehicles

Full Text:

PDF

References


Athanassiadis, B., Abbott, P. V., George, N., & Walsh, L. J. (2009). An in vitro study of the antimicrobial activity of some endodontic medicaments and their bases using an agar well diffusion assay. Aust Dent J, 54(2), 141-146. doi:10.1111/j.1834-7819.2009.01107.x

Ballal, V., Kundabala, M., Acharya, S., & Ballal, M. (2007). Antimicrobial action of calcium hydroxide, chlorhexidine and their combination on endodontic pathogens. Aust Dent J, 52(2), 118-121. doi:10.1111/j.1834-7819.2007.tb00475.x

Delattin, N., Cammue, B. P., & Thevissen, K. (2014). Reactive oxygen species-inducing antifungal agents and their activity against fungal biofilms. Future Med Chem, 6(1), 77-90. doi:10.4155/fmc.13.189

El Karim, I., Kennedy, J., & Hussey, D. (2007). The antimicrobial effects of root canal irrigation and medication. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 103(4), 560-569. doi:10.1016/j.tripleo.2006.10.004

Elsaka, S. E., & Elnaghy, A. M. (2012). Antibacterial activity of calcium hydroxide combined with chitosan solutions and the outcomes on the bond strength of RealSeal sealer to radicular dentin. J Biomed Res, 26(3), 193-199. doi:10.7555/jbr.26.20110136

Haapasalo, M., Shen, Y., Wang, Z., & Gao, Y. (2014). Irrigation in endodontics. Br Dent J, 216(6), 299-303. doi:10.1038/sj.bdj.2014.204

Hancock, H. H., 3rd, Sigurdsson, A., Trope, M., & Moiseiwitsch, J. (2001). Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91(5), 579-586. doi:10.1067/moe.2001.113587

Kim, D., & Kim, E. (2014). Antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: a literature review - Part I. In vitro studies. Restorative dentistry & endodontics, 39(4), 241-252. doi:10.5395/rde.2014.39.4.241

Liu, H., Wei, X., Ling, J., Wang, W., & Huang, X. (2010). Biofilm formation capability of Enterococcus faecalis cells in starvation phase and its susceptibility to sodium hypochlorite. J Endod, 36(4), 630-635. doi:10.1016/j.joen.2009.11.016

Love, R. M. (2001). Enterococcus faecalis--a mechanism for its role in endodontic failure. Int Endod J, 34(5), 399-405. doi:10.1046/j.1365-2591.2001.00437.x

Maekawa, L. E., Valera, M. C., Oliveira, L. D. d., Carvalho, C. A. T., Camargo, C. H. R., & Jorge, A. O. C. (2013). Effect of Zingiber officinale and propolis on microorganisms and endotoxins in root canals. Journal of applied oral science : revista FOB, 21(1), 25-31. doi:10.1590/1678-7757201302129

Maniglia-Ferreira, C., de Almeida-Gomes, F., Pinto, M. M., de Sousa Barbosa, F. T., de Farias Filho, D. M., & Albuquerque, N. L. (2016). In vitro evaluation of the antimicrobial effects of different intracanal medications in necrotic immature teeth. Eur Arch Paediatr Dent, 17(4), 251-255. doi:10.1007/s40368-016-0236-x

Mohammadi, Z., & Abbott, P. V. (2009). The properties and applications of chlorhexidine in endodontics. Int Endod J, 42(4), 288-302. doi:10.1111/j.1365-2591.2008.01540.x

Mozayeni, M. A., Hadian, A., Bakhshaei, P., & Dianat, O. (2015). Comparison of Antifungal Activity of 2% Chlorhexidine, Calcium Hydroxide, and Nanosilver gels against Candida Albicans. Journal of dentistry (Tehran, Iran), 12(2), 109-117.

Negi, P. S., Jayaprakasha, G. K., Jagan Mohan Rao, L., & Sakariah, K. K. (1999). Antibacterial activity of turmeric oil: a byproduct from curcumin manufacture. J Agric Food Chem, 47(10), 4297-4300. doi:10.1021/jf990308d

Pavaskar, R., de Ataide Ide, N., Chalakkal, P., Pinto, M. J., Fernandes, K. S., Keny, R. V., & Kamath, A. (2012). an in vitro study comparing the intracanal effectiveness of calcium hydroxide- and linezolid-based medicaments against Enterococcus faecalis. J Endod, 38(1), 95-100. doi:10.1016/j.joen.2011.09.031

Portenier, I., Waltimo, T., Orstavik, D., & Haapasalo, M. (2005). The susceptibility of starved, stationary phase, and growing cells of Enterococcus faecalis to endodontic medicaments. J Endod, 31(5), 380-386.

Raafat, D., & Sahl, H. G. (2009). Chitosan and its antimicrobial potential--a critical literature survey. Microb Biotechnol, 2(2), 186-201. doi:10.1111/j.1751-7915.2008.00080.x

Saatchi, M., Shokraneh, A., Navaei, H., Maracy, M. R., & Shojaei, H. (2014). Antibacterial effect of calcium hydroxide combined with chlorhexidine on Enterococcus faecalis: a systematic review and meta-analysis. Journal of applied oral science : revista FOB, 22(5), 356-365. doi:10.1590/1678-775720140032

Siqueira, J. F., Jr., & Lopes, H. P. (1999). Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J, 32(5), 361-369. doi:10.1046/j.1365-2591.1999.00275.x


Refbacks

  • There are currently no refbacks.